Product nameFluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue)
DescriptionA Fluo-4 analogue with lower affinity for calcium for detecting intracellular calcium levels. Cell-permeable.
- Fluo-5F AM
- Fluo5F, AM
Fluo-5 AM is an analogue of Fluo-4 with reduced calcium binding affinity (Kd = 2.3 μM). Suitable for detecting intracellular calcium levels in the range 1 μM to 1 mM. Cells can be loaded with Fluo-5 AM by adding the dissolved indicator directly to cells in culture dishes.
Fluo-5 AM has excitation and emission wavelengths at 494 and 516 nm respectively. Compatible with excitation by argon-ion lasers at 488 nm. Fluorescence inceases by >100-fold upon binding calcium.
4-(6-Acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4′-fluoro-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
Storage instructionsShipped at Room Temperature. Store at -20°C. Store In the Dark. Store under desiccating conditions.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Tran V et al. An improved measurement of the Ca2+-binding affinity of fluorescent Ca2+ indicators. Cell Calcium 71:86-94 (2018). Read more (PubMed: 29604967) »
- Wu Y et al. Calreticulin regulates TGF-ß1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling. Int J Biochem Cell Biol 90:103-113 (2017). Read more (PubMed: 28778674) »
- Eaddy AC & Schnellmann RG Visualization and quantification of endoplasmic reticulum Ca2+ in renal cells using confocal microscopy and Fluo5F. Biochem Biophys Res Commun 404:424-7 (2011). Read more (PubMed: 21130732) »