Overview

  • Product name
    Fluo-8 AM, green fluorescent calcium binding dye
  • Description
    Medium affinity green fluorescent calcium binding dye. Cell-permeable.
  • Biological description

    Medium affinity green fluorescent calcium binding dye. Binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding. Cell-permeable.

    Fluo-8 (or Fluo-2 Medium Affinity) has been found to be brighter (1.5x) than Fluo-4 in cellular experiments. It offers improved cell loading and Ca2+ response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at 490 nm and maximum emission at 520 nm. Fluo-8 loading can be performed at room temperature.

Properties

Images

  • U2OS cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom costar plate. The growth medium was removed, and the cells were incubated with 100 µl of 4 µM Fluo-4 AM or Fluo-8® AM in HHBS at 37 °C, 5% CO2 incubator for 1 hour. The cells were washed twice with 200 µl HHBS, then imaged with a fluorescence microscope using FITC channel.

  • Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol.

References

This product has been referenced in:
  • Hagen BM  et al. A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes. Cell Calcium 52:170-81 (2012). Read more (PubMed: 22721780) »
  • Del Negro CA  et al. Dendritic calcium activity precedes inspiratory bursts in preBotzinger complex neurons. J Neurosci 31:1017-22 (2011). Read more (PubMed: 21248126) »
  • Last NB  et al. Islet amyloid polypeptide demonstrates a persistent capacity to disrupt membrane integrity. Proc Natl Acad Sci U S A 108:9460-5 (2011). Read more (PubMed: 21606325) »
See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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