Fluorescein 5-maleimide, Fluorescent protein labeling reagent (ab145102)

Overview

  • Product name

    Fluorescein 5-maleimide, Fluorescent protein labeling reagent
  • Description

    Fluorescent protein labeling reagent.
  • Alternative names

    • 1-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione
  • Biological description

    Activated fluorescent molecule used to conjugate fluorescein to proteins. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond.

  • CAS Number

    75350-46-8
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    494nm
  • Emission

    519nm
  • Chemical name

    1-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione
  • Molecular weight

    427.36
  • Molecular formula

    C24H13NO7
  • Storage instructions

    Store at -20°C. Store In the Dark. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
  • Solubility overview

    DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Muraki M Preparation of a functional fluorescent human Fas ligand extracellular domain derivative using a three-dimensional structure guided site-specific fluorochrome conjugation. Springerplus 5:997 (2016). Read more (PubMed: 27398274) »
  • Nanda JS & Lorsch JR Labeling of a protein with fluorophores using maleimide derivitization. Methods Enzymol 536:79-86 (2014). Read more (PubMed: 24423268) »
  • Giangregorio N  et al. Conformation-dependent accessibility of Cys-136 and Cys-155 of the mitochondrial rat carnitine/acylcarnitine carrier to membrane-impermeable SH reagents. Biochim Biophys Acta 1767:1331-9 (2007). Read more (PubMed: 17961500) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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