Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [4-4-20 (enhanced)] to Fluorescein
- Suitable for: Flow Cyt, WB
- Reacts with: Species independent
Product nameAnti-Fluorescein antibody [4-4-20 (enhanced)]
See all Fluorescein primary antibodies
DescriptionHuman monoclonal [4-4-20 (enhanced)] to Fluorescein
Specificityab206509 is a recombinant monoclonal antibody to Fluorescein which is specific for Fluorescein labelled proteins. It is a His-tagged, Human Fab fragment.
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Fluorescein conjugated to Bovine Serum Albumin (BSA). Fluorescein I-bovine serum albumin (FlI-BSA).
This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
This antibody is His tagged.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Proclin 300
Constituent: 99% PBS
Concentration information loading...
Purification notesPurified by Immobilized Metal Affinity Chromatography
Clone number4-4-20 (enhanced)
Light chain typekappa
Our Abpromise guarantee covers the use of ab206509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
ab206509 has been referenced in 2 publications.
- Chen H et al. Single-blinded, randomized, and controlled clinical trial evaluating the effects of Omega-3 fatty acids among septic patients with intestinal dysfunction: A pilot study. Exp Ther Med 14:1505-1511 (2017). PubMed: 28810616
- Jung S et al. Selection for improved protein stability by phage display. J Mol Biol 294:163-80 (1999). PubMed: 10556036