Recombinant

Recombinant Anti-Fluorescein antibody [4-4-20 (enhanced)] (ab206509)

Overview

  • Product name

    Anti-Fluorescein antibody [4-4-20 (enhanced)]
    See all Fluorescein primary antibodies
  • Description

    Human monoclonal [4-4-20 (enhanced)] to Fluorescein
  • Host species

    Human
  • Specificity

    ab206509 is a recombinant monoclonal antibody to Fluorescein which is specific for Fluorescein labelled proteins. It is a His-tagged, Human Fab fragment.
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Fluorescein conjugated to Bovine Serum Albumin (BSA). Fluorescein I-bovine serum albumin (FlI-BSA).

  • General notes

    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques. 

    This antibody is His tagged.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Proclin
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified by Immobilized Metal Affinity Chromatography
  • Clonality

    Monoclonal
  • Clone number

    4-4-20 (enhanced)
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References

This product has been referenced in:

  • Chen H  et al. Single-blinded, randomized, and controlled clinical trial evaluating the effects of Omega-3 fatty acids among septic patients with intestinal dysfunction: A pilot study. Exp Ther Med 14:1505-1511 (2017). Read more (PubMed: 28810616) »
  • Jung S  et al. Selection for improved protein stability by phage display. J Mol Biol 294:163-80 (1999). Read more (PubMed: 10556036) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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