Recombinant Anti-Fluorescein antibody [4-4-20 (enhanced)] (ab206515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [4-4-20 (enhanced)] to Fluorescein
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
Overview
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Product name
Anti-Fluorescein antibody [4-4-20 (enhanced)]
See all Fluorescein primary antibodies -
Description
Rabbit monoclonal [4-4-20 (enhanced)] to Fluorescein -
Host species
Rabbit -
Specificity
This antibody recognizes Fluorescein labelled proteins. -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Fluorescein conjugated to Bovine Serum Albumin (BSA). Fluorescein I-bovine serum albumin (FlI-BSA).
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General notes
This is a chimeric antibody created as part of a panel offering antibodies of the same specificity in different formats (species, isotype, subtype and modified versions) for use as isotype controls.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4-4-20 (enhanced) -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
Alternative names
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab206515 has not yet been referenced specifically in any publications.