Recombinant

Recombinant Anti-Fluorescein antibody [4-4-20 (enhanced)] (ab206518)

Overview

  • Product name

    Anti-Fluorescein antibody [4-4-20 (enhanced)]
    See all Fluorescein primary antibodies
  • Description

    Mouse monoclonal [4-4-20 (enhanced)] to Fluorescein
  • Host species

    Mouse
  • Specificity

    Fluorescein labeled proteins.
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Fluorescein conjugated to Bovine Serum Albumin (BSA). (FlI-BSA).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Proclin
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    4-4-20 (enhanced)
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References

ab206518 has not yet been referenced specifically in any publications.

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