Key features and details
- Mouse monoclonal [6A4] to Fluorescein
- Suitable for: WB, ELISA, ICC
- Reacts with: Species independent
- Isotype: IgM
Product nameAnti-Fluorescein antibody [6A4]
See all Fluorescein primary antibodies
DescriptionMouse monoclonal [6A4] to Fluorescein
Tested applicationsSuitable for: WB, ELISA, ICCmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to Fluorescein.
General notesHuman synaptosome associated protein 23 (SNAP23) MW is 27 kDa
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab6213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ELISA||1/2000 - 1/20000.|
|ICC||1/200 - 1/2000.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6213 has been referenced in 3 publications.
- Kurokawa K et al. Sensitization of ethanol-induced place preference as a result of up-regulation of type 1 inositol 1,4,5-trisphosphate receptors in mouse nucleus accumbens. J Neurochem : (2014). PubMed: 25219476
- Maheswaranathan M et al. Platelet-derived Growth Factor (PDGF) Regulates Slingshot Phosphatase Activity via Nox1-dependent Auto-dephosphorylation of Serine 834 in Vascular Smooth Muscle Cells. J Biol Chem 286:35430-7 (2011). IP . PubMed: 21857021
- Odagi I et al. Examination of normal intestine using confocal endomicroscopy. J Gastroenterol Hepatol 22:658-62 (2007). PubMed: 17444852