Overview

  • Product name

    Anti-Fluorescein antibody [6A4]
    See all Fluorescein primary antibodies
  • Description

    Mouse monoclonal [6A4] to Fluorescein
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IP, ICCmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Fluorescein.

  • General notes

    Human synaptosome associated protein 23 (SNAP23) MW is 27 kDa

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    6A4
  • Myeloma

    unknown
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/2000 - 1/20000.
IP Use at an assay dependent concentration. PubMed: 21857021
ICC 1/200 - 1/2000.

Target

  • Relevance

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References

This product has been referenced in:

  • Kurokawa K  et al. Sensitization of ethanol-induced place preference as a result of up-regulation of type 1 inositol 1,4,5-trisphosphate receptors in mouse nucleus accumbens. J Neurochem : (2014). Read more (PubMed: 25219476) »
  • Maheswaranathan M  et al. Platelet-derived Growth Factor (PDGF) Regulates Slingshot Phosphatase Activity via Nox1-dependent Auto-dephosphorylation of Serine 834 in Vascular Smooth Muscle Cells. J Biol Chem 286:35430-7 (2011). IP . Read more (PubMed: 21857021) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in indirect ELISA only.

Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments between antibodieswill not often have been done.

If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

I hope this will be helpful to you. Should you have any further questions please do not hesitate to ask.

Read More

Answer

Thank you for your enquiry. I am sorry that the answer has taken so long. The answer from the source of the antiserum has not been forthcoming. Unfortunately I have been informed that ab6213's cross reactivity with carboxyfluorescein has never been tested.

Read More

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