Key features and details
- Rabbit polyclonal to Fluorescein
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Fluorescein antibody
See all Fluorescein primary antibodies
DescriptionRabbit polyclonal to Fluorescein
SpecificitySpecific to Fluoresceine. No cross reactivity with immunoglobulin of any species.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Species independent
Fluoresceine conjugated to KHL.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography
Our Abpromise guarantee covers the use of ab19491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
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ab19491 has been referenced in 11 publications.
- Tamura T et al. Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide. Nat Commun 9:1870 (2018). PubMed: 29760386
- Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell 70:1038-1053.e7 (2018). PubMed: 29932899
- Takaoka Y et al. A rationally designed JAZ subtype-selective agonist of jasmonate perception. Nat Commun 9:3654 (2018). PubMed: 30194307
- Feng X et al. A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein a for solid tumor diagnosis. Int J Nanomedicine 12:5359-5372 (2017). PubMed: 28794628
- Koole C et al. Genetically encoded photocross-linkers determine the biological binding site of exendin-4 peptide in the N-terminal domain of the intact human glucagon-like peptide-1 receptor (GLP-1R). J Biol Chem 292:7131-7144 (2017). PubMed: 28283573