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Dear technical team,
Our customer would like to purchase Fluorescein antibody for ELISA.
Her plan is;
1. coating protein A on the well plate
2. coating the antibody on it.
3. incubate aptamer-fluorescein complex
4. incubate target molecule(which can bind to aptamer)
5. incubate biotin-some complex
6. incubate avdin-HRP complex
She searched one monoclonal antibody ab6213 and one polyclonal antibody ab19491.
Please let me know which one is better choice for our customer.
Asked on May 17 2012
Thank you for your enquiry.
I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in indirect ELISA only.
Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments between antibodieswill not often have been done.
If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).
I hope this will be helpful to you. Should you have any further questions please do not hesitate to ask.
Answered on May 17 2012