Anti-Fluorescein antibody (ab6644)

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Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/25000.
IHC-Fr 1/250 - 1/1500.

Target

  • Relevance
    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References

ab6644 has not yet been referenced specifically in any publications.

Publishing research using ab6644? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Today I have spoken on the phone to Danielle Miller about your Goat anti-fluoresceine AB6644. We do not have signal with the antibody, only an aspecific band. I have filled in a form via your website about the problems we have. Attached to this mail are two files with images of the blots we obtain: First:' blotabcam' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with your antibody (1:1000)and as secondary antibody we have used donkey anti goat with peroxidase(1:5000) from Jackson Immuno Research. Second: ' blotbiogenisis.jpg' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with rabbit anti fluoresceine conjugated with a peroxidase. The lot nr of our antibody is 8001. We would like to obtain another lot of the antibody to test again.

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Answer

Thank you for you e-mail and providing more details of your protocol. We are going to send you a new vial from a different batch (13597) - free of charge. For your information, the new order number is 42198. Please do let us know how you are gettin on this vial. Good luck!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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