Question (8267) | Anti-Fluorescein antibody (ab6644)

Go to datasheet (ab6644)

Question

Today I have spoken on the phone to Danielle Miller about your Goat anti-fluoresceine AB6644. We do not have signal with the antibody, only an aspecific band. I have filled in a form via your website about the problems we have. Attached to this mail are two files with images of the blots we obtain: First:' blotabcam' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with your antibody (1:1000)and as secondary antibody we have used donkey anti goat with peroxidase(1:5000) from Jackson Immuno Research. Second: ' blotbiogenisis.jpg' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with rabbit anti fluoresceine conjugated with a peroxidase. The lot nr of our antibody is 8001. We would like to obtain another lot of the antibody to test again.

Answer

Thank you for you e-mail and providing more details of your protocol. We are going to send you a new vial from a different batch (13597) - free of charge. For your information, the new order number is 42198. Please do let us know how you are gettin on this vial. Good luck!

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