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Today I have spoken on the phone to Danielle Miller about your Goat anti-fluoresceine AB6644. We do not have signal with the antibody, only an aspecific band. I have filled in a form via your website about the problems we have. Attached to this mail are two files with images of the blots we obtain: First:' blotabcam' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with your antibody (1:1000)and as secondary antibody we have used donkey anti goat with peroxidase(1:5000) from Jackson Immuno Research. Second: ' blotbiogenisis.jpg' which contains 3 samples and 2 markers. On the image should be clear that nr 1 is the negative control and 2 and 3 are the positive controls.This blot is detected with rabbit anti fluoresceine conjugated with a peroxidase. The lot nr of our antibody is 8001. We would like to obtain another lot of the antibody to test again.
Asked on May 19 2004
Thank you for you e-mail and providing more details of your protocol. We are going to send you a new vial from a different batch (13597) - free of charge. For your information, the new order number is 42198. Please do let us know how you are gettin on this vial. Good luck!
Answered on May 20 2004