Alkaline Phosphatase Anti-Fluorescein antibody (ab6657)
Key features and details
- Alkaline Phosphatase Goat polyclonal to Fluorescein
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-Fluorescein antibody
See all Fluorescein primary antibodies -
Description
Alkaline Phosphatase Goat polyclonal to Fluorescein -
Host species
Goat -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
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General notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000 - 1/5000.
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ELISA |
1/1000 - 1/5000.
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|
IHC-P |
1/1000 - 1/5000.
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|
IHC-Fr |
1/1000 - 1/5000.
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|
WB |
1/1000 - 1/5000.
|
Notes |
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Dot blot
1/1000 - 1/5000. |
ELISA
1/1000 - 1/5000. |
IHC-P
1/1000 - 1/5000. |
IHC-Fr
1/1000 - 1/5000. |
WB
1/1000 - 1/5000. |
Target
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Relevance
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
Alternative names
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab6657 has not yet been referenced specifically in any publications.