Key features and details
- Goat polyclonal to Fluorescein (Alkaline Phosphatase)
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Product nameAnti-Fluorescein antibody (Alkaline Phosphatase)
See all Fluorescein primary antibodies
DescriptionGoat polyclonal to Fluorescein (Alkaline Phosphatase)
Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Species independent
Fluorescein conjugated to Goat IgG
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab6657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||1/1000 - 1/5000.|
|ELISA||1/1000 - 1/5000.|
|IHC-P||1/1000 - 1/5000.|
|IHC-Fr||1/1000 - 1/5000.|
|WB||1/1000 - 1/5000.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
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ab6657 has not yet been referenced specifically in any publications.