Product nameAnti-Fluorescein antibody (Biotin)
See all Fluorescein primary antibodies
DescriptionGoat polyclonal to Fluorescein (Biotin)
Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details
Species reactivityReacts with: Species independent
Fluorescein conjugated to Goat IgG
General notesFor extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab6655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
|IHC-P||1/1000 - 1/5000.|
|IHC-Fr||1/1000 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|In situ hybridization||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Anti-Fluorescein antibody (Biotin) (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.
Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution
Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
This product has been referenced in:
- He B et al. Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules. J Pathol 245:209-221 (2018). Read more (PubMed: 29603739) »
- Long K et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl . Read more (PubMed: 26838601) »