Key features and details
- Mouse monoclonal [BT.6A4] to Fluorescein (Biotin)
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgM
Product nameAnti-Fluorescein antibody [BT.6A4] (Biotin)
See all Fluorescein primary antibodies
DescriptionMouse monoclonal [BT.6A4] to Fluorescein (Biotin)
SpecificityThe antibody reacts with free and bound fluorescein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details
Species reactivityReacts with: Species independent
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified anti-fluorescein biotin conjugate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab6215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ELISA||1/1000 - 1/10000.|
|IHC-P||1/200 - 1/2000.|
|IHC-Fr||1/200 - 1/2000.|
|In situ hybridization||Use at an assay dependent dilution.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
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ab6215 has been referenced in 1 publication.
- Mukaiyama M et al. Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation. J Biochem N/A:N/A (2020). PubMed: 32428205