Overview

  • Product name

    Anti-Fluorescein antibody [BT.6A4] (Biotin)
    See all Fluorescein primary antibodies
  • Description

    Mouse monoclonal [BT.6A4] to Fluorescein (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    The antibody reacts with free and bound fluorescein.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details
  • Species reactivity

    Reacts with: Species independent

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purification notes

    Purified anti-fluorescein biotin conjugate.
  • Clonality

    Monoclonal
  • Clone number

    BT.6A4
  • Myeloma

    unknown
  • Isotype

    IgM
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
In situ hybridization Use at an assay dependent dilution.

Target

  • Relevance

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References

ab6215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

You could detect the biotinylated antibody directly or use a secondary to the fluorescein antibody.

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Answer

This antibody has not been tested in flow cytometry. However, in theory it should work in your application.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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