Product nameAnti-Fluorescein antibody (HRP)
See all Fluorescein primary antibodies
DescriptionRabbit polyclonal to Fluorescein (HRP)
SpecificitySpecific to Fluoresceine.
Tested applicationsSuitable for: IP, IHC-P, WB, ELISAmore details
Species reactivityReacts with: Species independent
Fluorescein coupled to KLH
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA
Concentration information loading...
Purification notesSolid phase hapten antibody capture. Affinity purified and absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated substitution ratio: HRP:IgG is >1.5
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab19492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 19706527|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/20 - 1/200.|
|ELISA||1/200 - 1/1000.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Immunohistochemical analysis of chicken trachea tissue, infected with an avian influenza virus, staining Fluorescein with ab19492.
Fluoroscein-labeled influenza viruses were incubated with tissue overnight at 4°C. Sections were incubated with primary antibody and the signal was amplified with a tyramide signal amplification system.
This product has been referenced in:
- Eriksson P et al. Characterization of avian influenza virus attachment patterns to human and pig tissues. Sci Rep 8:12215 (2018). Read more (PubMed: 30111851) »
- Hasegawa K et al. Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. PLoS One 12:e0172030 (2017). Read more (PubMed: 28182792) »