HRP Anti-Fluorescein antibody (ab6656)
Key features and details
- HRP Goat polyclonal to Fluorescein
- Suitable for: ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Fluorescein antibody
See all Fluorescein primary antibodies -
Description
HRP Goat polyclonal to Fluorescein -
Host species
Goat -
Conjugation
HRP -
Specificity
This antibody reacts with FITC-conjugated proteins. -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/200000.
|
|
IHC-P | (1) |
1/500 - 1/2500.
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
WB |
1/1000 - 1/5000.
|
Notes |
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ELISA
1/200000. |
IHC-P
1/500 - 1/2500. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. |
Target
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Relevance
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
Alternative names
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Images
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Immunohistochemical analysis of Mouse thymus in which the medullary epithellai cells are labelled with fluorescein-conjugated UEA1 lectin with ab6656. Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 40 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C.
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ab6656 staining GFP in Mouse mammary carcinoma metastasis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Perfusion fixed tissue with 4% paraformaldehyde, blocked with 0.5% Perkin-Elmer TNB blocking reagent for 30 minutes at 25°C and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500) for 30 minutes at 25°C
Micrograph demonstrates use of ab6662 for detection of GFP-positive tumor cells (LEFT) and an adjacent section after amplification with ab6656 (RIGHT). Images were taken with identical settings on a Leica confocal microscope.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab6656 has been referenced in 7 publications.
- Healing E et al. A panel of colorimetric assays to measure enzymatic activity in the base excision DNA repair pathway. Nucleic Acids Res 47:e61 (2019). PubMed: 30869144
- Shigdar S et al. Application of Aptamers in Histopathology. Methods Mol Biol 1380:191-6 (2016). PubMed: 26552827
- de Lucas M et al. Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell-Type Proliferation and Differentiation. Plant Cell 28:2616-2631 (2016). In situ hybridization ; Arabidopsis thaliana . PubMed: 27650334
- Lin WR et al. The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis. Biomed Res Int 2016:4601532 (2016). PubMed: 27314021
- Xiang D et al. Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors. Theranostics 5:1083-97 (2015). IHC . PubMed: 26199647
- Shigdar S et al. The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One 8:e57613 (2013). IHC-P ; Human . PubMed: 23460885
- Kett LR et al. LRRK2 Parkinson disease mutations enhance its microtubule association. Hum Mol Genet 21:890-9 (2012). PubMed: 22080837