Overview

  • Product name

    Fluorescent Western Blot Kit
  • Product overview

    Optiblot Fluorescent Western Blot Kit allows the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a loading control alongside a protein of interest. Assay phosphorylated and non-phosphorylated isoforms of a protein simultaneously. The Optiblot Fluorescent Western Blot Kit protocol saves time and money since there is no need to strip and re-probe a blot, no use of disposable film, and the blot can be imaged immediately, without drying.

    The fluorescent dyes provided with the Optiblot Fluorescent Western Blot Kit outperform Cy dyes and allow detection of low pg amounts of protein.

     

    Compatible with CCD imagers equipped with Cy3(Green)/Cy5(Red) excitation and emission. (e.g. ChemiDoc MP, LAS 4000, Fluorchem Q and M systems, G:BOX, Gel Logic, Fusion FX5, Typhoon imager, etc)

  • Tested applications

    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Images

  • Simultaneous detection of EGFR and phospho-EGFR with Obtiblot Fluorescent Western Blot Kit. The increase in phosphorylation of EGFR in response to EGF was detected. Lysates from A431 cells (lane 1) and from A431 cells treated with EGF (lane 2) were blotted and EGFR detected in the green channel (b) or phospho-EGFR detected in the red channel (c). The two channels are superimposed in (a). Lane 3: molecular weight markers.
  • Optiblot Conjugates provide a brighter signal than the ECL Plex Western Blotting system. Duplicate Western blots containing samples of AFP and CEA proteins were treated identically and probed with the same mixture of mouse anti-AFP and rabbit anti-CEA primary antibodies. One blot was then stained with Optiblot conjugates (a) and the other with and identical concentration of Cy3 anti-mouse and Cy5 anti-rabbit antibodies (b). Under identical imaging conditions, Optiblot provides a brighter signal, and 10x greater sensitivity.

Protocols

References

ab133410 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. I hope that you are well.

The ab133410 is a fluorescence based kit however the Roche kit cat # 11520709001 is a chemiluminescence based so this will not be a right alternative.

Please suggest ab133406, https://www.abcam.com/Optiblot-ECL-Detect-Kit-23pg-187ng-ab133406.html

This kit is vigorously tested and is fully guaranteed. Other alternatives are ab65623, ab65628, ab133408 and ab133409.

Please note that the kits ab133406 does not have secondary antibody as the Roche kit. However all HRP conjugated secondary would be suitable.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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