Product nameFluorescent Western Blot Kit
Optiblot Fluorescent Western Blot Kit allows the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a loading control alongside a protein of interest. Assay phosphorylated and non-phosphorylated isoforms of a protein simultaneously. The Optiblot Fluorescent Western Blot Kit protocol saves time and money since there is no need to strip and re-probe a blot, no use of disposable film, and the blot can be imaged immediately, without drying.
The fluorescent dyes provided with the Optiblot Fluorescent Western Blot Kit outperform Cy dyes and allow detection of low pg amounts of protein.
Compatible with CCD imagers equipped with Cy3(Green)/Cy5(Red) excitation and emission. (e.g. ChemiDoc MP, LAS 4000, Fluorchem Q and M systems, G:BOX, Gel Logic, Fusion FX5, Typhoon imager, etc)
Tested applicationsSuitable for: WBmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 tests APC-goat-anti-rabbit IgG antibody 1 x 50µl Background Quenching Sheets 10 units Immunoblot washing solution, 10x concentrate 1 x 120ml ab133411 - Low-fluorescence PVDF transfer membrane 9x7 cm 10 units Protein-free blocking solution, 5x concentrate 1 x 50ml RPE-goat-anti-mouse IgG antibody 1 x 50µl
- Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa) (ab115832)
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (ab116029)
- TEO-Tricine Precast Gels - RunBlue™ (4-20%, 12-well, 8x10cm) (ab119209)
- InstantBlue® Coomassie Protein Stain (ab119211)
- Bradford Reagent (ab119216)
- ECL Substrate Kit (High Sensitivity) (ab133406)
Our Abpromise guarantee covers the use of ab133410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
Simultaneous detection of EGFR and phospho-EGFR with Obtiblot Fluorescent Western Blot Kit. The increase in phosphorylation of EGFR in response to EGF was detected. Lysates from A431 cells (lane 1) and from A431 cells treated with EGF (lane 2) were blotted and EGFR detected in the green channel (b) or phospho-EGFR detected in the red channel (c). The two channels are superimposed in (a). Lane 3: molecular weight markers.
Optiblot Conjugates provide a brighter signal than the ECL Plex Western Blotting system. Duplicate Western blots containing samples of AFP and CEA proteins were treated identically and probed with the same mixture of mouse anti-AFP and rabbit anti-CEA primary antibodies. One blot was then stained with Optiblot conjugates (a) and the other with and identical concentration of Cy3 anti-mouse and Cy5 anti-rabbit antibodies (b). Under identical imaging conditions, Optiblot provides a brighter signal, and 10x greater sensitivity.
ab133410 has not yet been referenced specifically in any publications.