Key features and details
- Rabbit polyclonal to FMN2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FMN2 antibody
See all FMN2 primary antibodies
DescriptionRabbit polyclonal to FMN2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (Human) corresponding to a region between residues 650-700 of human FMN2. (NP_064450.3).
- human brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab72052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation.
Tissue specificityExpressed almost exclusively in the developing and mature central nervous system.
Sequence similaritiesBelongs to the formin homology family. Cappuccino subfamily.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cytoplasm > cytosol. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm > cell cortex. Nucleus > nucleolus. Recruited to the membranes via its interaction with SPIRE1. Colocalizes with the actin cytoskeleton. Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion.
- Information by UniProt
- FMN 2 antibody
- Fmn2 antibody
- FMN2_HUMAN antibody
All lanes : Anti-FMN2 antibody (ab72052) at 0.25 µg/ml
Lane 1 : human brain lysate
Lane 2 : human brain lysate with immunising peptide
Predicted band size: 180 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 67 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue section staining FMN2 using ab72052 at 1/300 dilution. HRP- labeled was used as the secondary antibody and hematoxylin was used as a nuclei counterstain.
ab72052 has been referenced in 4 publications.
- Xie B et al. Poly(ADP-ribose) mediates asymmetric division of mouse oocyte. Cell Res 28:462-475 (2018). PubMed: 29463901
- Yang Z et al. Nuclear Envelope Rupture Is Enhanced by Loss of p53 or Rb. Mol Cancer Res 15:1579-1586 (2017). PubMed: 28811362
- Skau CT et al. FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis. Cell 167:1571-1585.e18 (2016). PubMed: 27839864
- Lynch J et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . PubMed: 24223803