Anti-FMN2 antibody - C-terminal (ab226944)
Key features and details
- Rabbit polyclonal to FMN2 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FMN2 antibody - C-terminal
See all FMN2 primary antibodies -
Description
Rabbit polyclonal to FMN2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human FMN2 (C terminal). The exact sequence is proprietary.
Database link: Q9NZ56 -
Positive control
- WB: K562 and THP-1 whole cell extracts; human brain tissue extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 180 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 180 kDa. |
Target
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Function
Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation. -
Tissue specificity
Expressed almost exclusively in the developing and mature central nervous system. -
Sequence similarities
Belongs to the formin homology family. Cappuccino subfamily.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cytoplasm > cytosol. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm > cell cortex. Nucleus > nucleolus. Recruited to the membranes via its interaction with SPIRE1. Colocalizes with the actin cytoskeleton. Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion. - Information by UniProt
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Database links
- Entrez Gene: 56776 Human
- Omim: 606373 Human
- SwissProt: Q9NZ56 Human
- Unigene: 24889 Human
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Alternative names
- FMN 2 antibody
- Fmn2 antibody
- FMN2_HUMAN antibody
see all
Images
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All lanes : Anti-FMN2 antibody - C-terminal (ab226944) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell extract
Lane 4 : Human brain extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 180 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226944 has not yet been referenced specifically in any publications.