Overview

  • Product name
    Anti-FMNL1 antibody [2369E4a]
    See all FMNL1 primary antibodies
  • Description
    Mouse monoclonal [2369E4a] to FMNL1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    FMNL1 recombinant fragment (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab54361 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2369E4a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 122 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
  • Tissue specificity
    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the formin homology family.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Domain
    The DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
  • Post-translational
    modifications
    Myristoylation mediates membrane localization and blebbing.
  • Cellular localization
    Cytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8030453N10Rik antibody
    • AI553564 antibody
    • C17orf1 antibody
    • C17orf1B antibody
    • CLL associated antigen KW 13 antibody
    • CLL-associated antigen KW-13 antibody
    • FHOD4 antibody
    • FMNL antibody
    • FMNL_HUMAN antibody
    • Fmnl1 antibody
    • Fnrl antibody
    • Formin like 1 antibody
    • Formin like antibody
    • Formin related protein antibody
    • Formin-like protein 1 antibody
    • Frls antibody
    • KW 13 antibody
    • KW13 antibody
    • Leukocyte formin antibody
    • MGC133052 antibody
    • MGC1894 antibody
    • MGC21878 antibody
    see all

Images

  • Anti-FMNL1 antibody [2369E4a] (ab54361) + immunizing recombinant protein

    Predicted band size: 122 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.

References

ab54361 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab54361.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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