Anti-FMNL1 antibody (ab97456)
Key features and details
- Rabbit polyclonal to FMNL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FMNL1 antibody
See all FMNL1 primary antibodies -
Description
Rabbit polyclonal to FMNL1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 631 - 873 of Human FMNL1 (NP_005883).
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Positive control
- 293T, A431, HepG2, MOLT4 and Raji cell lysates. H1299 and HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa. |
Target
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Function
May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the formin homology family.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
Domain
The DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments. -
Post-translational
modificationsMyristoylation mediates membrane localization and blebbing. -
Cellular localization
Cytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1. - Information by UniProt
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Database links
- Entrez Gene: 752 Human
- Entrez Gene: 57778 Mouse
- Omim: 604656 Human
- SwissProt: O95466 Human
- SwissProt: Q9JL26 Mouse
- Unigene: 100217 Human
- Unigene: 138913 Mouse
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Alternative names
- 8030453N10Rik antibody
- AI553564 antibody
- C17orf1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97456 has been referenced in 2 publications.
- Kage F et al. FMNL formins boost lamellipodial force generation. Nat Commun 8:14832 (2017). PubMed: 28327544
- Monzo P et al. Mechanical confinement triggers glioma linear migration dependent on formin FHOD3. Mol Biol Cell 27:1246-61 (2016). Rat . PubMed: 26912794