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    fmnl2-antibody-ab241279.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other
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Anti-FMNL2 antibody (ab241279)

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Abpromise

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Immunoprecipitation - Anti-FMNL2 antibody (ab241279)

    Key features and details

    • Rabbit polyclonal to FMNL2
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-FMNL2 antibody
      See all FMNL2 primary antibodies
    • Description

      Rabbit polyclonal to FMNL2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide within Human FMNL2 aa 425-475. The exact sequence is proprietary.
      Database link: Q96PY5

    • Positive control

      • IP: HeLa whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab241279 was affinity purified using an epitope specific to FMNL2 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • Small G Proteins
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)

    Applications

    Our Abpromise guarantee covers the use of ab241279 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at 2-10 µg/mg of lysate.

    Target

    • Relevance

      This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis, cytokinesis, and cell polarity. Alternatively spliced transcript variants encoding different isoforms have been described but their full-length nature has yet to be determined.
    • Cellular localization

      Cytoplasm
    • Database links

      • Entrez Gene: 114793 Human
      • Entrez Gene: 71409 Mouse
      • Omim: 616285 Human
      • SwissProt: Q96PY5 Human
      • SwissProt: A2APV2 Mouse
      • Unigene: 654630 Human
      • Alternative names

        • FHOD2 antibody
        • Formin homology 2 domain containing protein 2 antibody
        • Formin like 2 antibody
        • Formin like protein 2 antibody
        • KIAA1902 antibody
        see all

      Images

      • Immunoprecipitation - Anti-FMNL2 antibody (ab241279)
        Immunoprecipitation - Anti-FMNL2 antibody (ab241279)

        FMNL2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241279 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241279 at 0.4 µg/ml.

        Lane 1: ab241279 IP in HeLa whole cell lysate.
        Lane 2: Control IgG IP in HeLa whole cell lysate.

        Detection: Chemiluminescence with exposure time of 10 seconds.

        Lysates prepared in NETN buffer.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab241279? Please let us know so that we can cite the reference in this datasheet.

      ab241279 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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