Anti-FMO3 antibody (ab154089)
Key features and details
- Rabbit polyclonal to FMO3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FMO3 antibody
See all FMO3 primary antibodies -
Description
Rabbit polyclonal to FMO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment corresponding to a region within amino acids 270-519 of Human FMO3 (UniProt ID: P31513).
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Positive control
- 293T, A431, H1299, HeLa, Molt4, Raji and mouse liver whole cell lysates; A431 cells; OVCAR3 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds. -
Tissue specificity
Liver. -
Involvement in disease
Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine. -
Sequence similarities
Belongs to the FMO family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2328 Human
- Entrez Gene: 14262 Mouse
- Omim: 136132 Human
- SwissProt: P31513 Human
- SwissProt: P97501 Mouse
- Unigene: 445350 Human
- Unigene: 2900 Mouse
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Alternative names
- Dimethylaniline monooxygenase [N oxide forming] 3 antibody
- Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
- Dimethylaniline monooxygenase 3 antibody
see all
Images
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Western blot - Anti-FMO3 antibody (ab154089)Anti-FMO3 antibody (ab154089) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Western blot - Anti-FMO3 antibody (ab154089)Anti-FMO3 antibody (ab154089) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody (ab154089)Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft tissue labeling FMO3 with ab154089 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody (ab154089)Immunofluorescence analysis of methanol-fixed A431 cells labeling FMO3 with ab154089 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.
Protocols
References (0)
ab154089 has not yet been referenced specifically in any publications.
