Anti-FMO3 antibody (ab154089)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FMO3 antibody
See all FMO3 primary antibodies -
Description
Rabbit polyclonal to FMO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment corresponding to a region within amino acids 270-519 of Human FMO3 (UniProt ID: P31513).
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Positive control
- 293T, A431, H1299, HeLa, Molt4, Raji and mouse liver whole cell lysates; A431 cells; OVCAR3 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds. -
Tissue specificity
Liver. -
Involvement in disease
Defects in FMO3 are the cause of trimethylaminuria (TMAU) [MIM:602079]; also known as fish-odor syndrome. TMAU is an inborn error of metabolism associated with an offensive body odor and caused by deficiency of FMO-mediated N-oxidation of amino-trimethylamine (TMA) derived from foodstuffs. Such individuals excrete relatively large amounts of TMA in their urine, sweat, and breath, and exhibit a fishy body odor characteristic of the malodorous free amine. -
Sequence similarities
Belongs to the FMO family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2328 Human
- Entrez Gene: 14262 Mouse
- Omim: 136132 Human
- SwissProt: P31513 Human
- SwissProt: P97501 Mouse
- Unigene: 445350 Human
- Unigene: 2900 Mouse
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Alternative names
- Dimethylaniline monooxygenase [N oxide forming] 3 antibody
- Dimethylaniline monooxygenase [N-oxide-forming] 3 antibody
- Dimethylaniline monooxygenase 3 antibody
see all
Images
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Western blot - Anti-FMO3 antibody (ab154089)Anti-FMO3 antibody (ab154089) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Western blot - Anti-FMO3 antibody (ab154089)Anti-FMO3 antibody (ab154089) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO3 antibody (ab154089)Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft tissue labeling FMO3 with ab154089 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-FMO3 antibody (ab154089)Immunofluorescence analysis of methanol-fixed A431 cells labeling FMO3 with ab154089 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.
Protocols
References
ab154089 has not yet been referenced specifically in any publications.
