Recombinant Anti-FMO5 antibody [EPR14783] (ab189516)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14783] to FMO5
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FMO5 antibody [EPR14783]
See all FMO5 primary antibodies -
Description
Rabbit monoclonal [EPR14783] to FMO5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver tissue lysate and HepG2 cell lysate. IHC-P: Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14783 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/50.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/50. |
Target
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Function
In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. -
Tissue specificity
Expressed in fetal and adult liver. -
Sequence similarities
Belongs to the FMO family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2330 Human
- Entrez Gene: 14263 Mouse
- Omim: 603957 Human
- SwissProt: P49326 Human
- SwissProt: P97872 Mouse
- Unigene: 642706 Human
- Unigene: 385180 Mouse
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Alternative names
- Dimethylaniline monooxygenase [N oxide forming] 5 antibody
- Dimethylaniline monooxygenase [N-oxide-forming] 5 antibody
- Dimethylaniline oxidase 5 antibody
see all
Images
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Anti-FMO5 antibody [EPR14783] (ab189516) at 1/10000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Anti-FMO5 antibody [EPR14783] (ab189516) at 1/10000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Immunohistochemical analysis of paraffine embedded Human liver tissue labeling FMO5 with ab189516 at 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunoprecipitation of Human fetal liver labeling FMO5 with ab189516 at 1/50 diltion and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 (+) or PBS (-)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab189516 has been referenced in 1 publication.
- Zhang T et al. Overexpression of flavin-containing monooxygenase 5 predicts poor prognosis in patients with colorectal cancer. Oncol Lett 15:3923-3927 (2018). PubMed: 29456741