Key features and details
- Mouse monoclonal [1D10] to FMRP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-FMRP antibody [1D10]
See all FMRP primary antibodies
DescriptionMouse monoclonal [1D10] to FMRP
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
Recombinant fragment corresponding to Human FMRP aa 36-279. Produced in E. coli.(NP_002015).
WQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVR MIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPE DLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLI DMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIG THGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFL
Database link: Q06787
- WB: Untransfected and pCMV6-ENTRY FMRP-transfected HEK-293T whole cell lysate. IHC-P: Human lung tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab230915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 71 kDa.|
FunctionTranslation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
Tissue specificityHighest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
Involvement in diseaseDefects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the FMR1 family.
Contains 2 KH domains.
modificationsPhosphorylated on several serine residues.
Cellular localizationCytoplasm. Nucleus > nucleolus.
- Information by UniProt
- FMR 1 antibody
- Fmr1 antibody
- Fmr1 gene antibody
Formalin-fixed, paraffin-embedded human lung tissue stained for FMRP using ab230915 at 10 μg/ml in immunohistochemical analysis.
All lanes : Anti-FMRP antibody [1D10] (ab230915) at 1/500 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY FMRP-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 71 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230915 has not yet been referenced specifically in any publications.