Anti-FMRP antibody [1D10] (ab230915)
Key features and details
- Mouse monoclonal [1D10] to FMRP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-FMRP antibody [1D10]
See all FMRP primary antibodies -
Description
Mouse monoclonal [1D10] to FMRP -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan -
Immunogen
Recombinant fragment corresponding to Human FMRP aa 1-300. Produced in E. coli.(NP_002015).
Database link: Q06787 -
Positive control
- WB: Untransfected and pCMV6-ENTRY FMRP-transfected HEK-293T whole cell lysate. IHC-P: Human lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1D10 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/2000. Predicted molecular weight: 71 kDa.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/2000. Predicted molecular weight: 71 kDa. |
Target
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Function
Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). -
Tissue specificity
Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. -
Involvement in disease
Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the FMR1 family.
Contains 2 KH domains. -
Post-translational
modificationsPhosphorylated on several serine residues. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 2332 Human
- Entrez Gene: 14265 Mouse
- Entrez Gene: 24948 Rat
- Omim: 309550 Human
- SwissProt: Q06787 Human
- SwissProt: P35922 Mouse
- SwissProt: Q80WE1 Rat
- Unigene: 103183 Human
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Alternative names
- FMR 1 antibody
- Fmr1 antibody
- Fmr1 gene antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung tissue stained for FMRP using ab230915 at 10 μg/ml in immunohistochemical analysis.
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All lanes : Anti-FMRP antibody [1D10] (ab230915) at 1/500 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY FMRP-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 71 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230915 has been referenced in 1 publication.
- Zhan X et al. FMRP(1-297)-tat restores ion channel and synaptic function in a model of Fragile X syndrome. Nat Commun 11:2755 (2020). PubMed: 32488011