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Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain sections)
Antigen retrieval step
Other product details
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3% Triton X100
This antibody produces a strong staining in tracts of fibers in many brain areas such as the striatum (picture attached). The staining does not seem specific.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Thank you for your honest and valuable feedback. Abcam has not tested this antibody in IHC-FoFr or mouse, and your data suggest it may not be suitable for this application and/or species.
Dr. Sophie Pezet
Submitted Jan 28 2009