Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-FMRP antibody

Poor
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3% Triton X100

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
This antibody produces a strong staining in tracts of fibers in many brain areas such as the striatum (picture attached). The staining does not seem specific.

The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Abcam response
Thank you for your honest and valuable feedback. Abcam has not tested this antibody in IHC-FoFr or mouse, and your data suggest it may not be suitable for this application and/or species.

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

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