Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-FMRP antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Other product details

Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3% Triton X100

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
This antibody produced a nice nuclear staining in many brain areas. The picture shows the staining obtained in the rat cortex. The picture was taken using the objective X40.

The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

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