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Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Brain sections)
Antigen retrieval step
Other product details
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3% Triton X100
This antibody produced a nice nuclear staining in many brain areas. The picture shows the staining obtained in the rat cortex. The picture was taken using the objective X40.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Dr. Sophie Pezet
Submitted Jan 28 2009