Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FMRP antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain: frontal cortex)
Specification
Brain: frontal cortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/1500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
An excellent pattern of immunostaining.
I am not familiar with the distribution pattern of this protein, but I see strong granular somatic positivity and also a dendritic field single granule postivity that appears to be associated with fine, positive nerve fibres ( submitted image of adult mouse - red arrowheads).
Both granular patterns appear to be very similar to published results for FMRP distribution in rat cortex however I have not, in a limited reading, observed any nerve fibre positivity in any area of adult rat brain, identically immunostained.

Mr. Carl Hobbs

Verified customer

Submitted Sep 01 2010

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