Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (megakaryocytes)
Specification
megakaryocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ecl
Bands
Specific: 71 kDa
Exposure
5 minute(s) and 0 second(s)

Additional data

Additional Notes
In the image you can see the result with 10 and 30 ug of total proteins.

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Submitted May 29 2013

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