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  1. Link

    fmrp-phospho-s499-antibody-ab183319.pdf

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Anti-FMRP (phospho S499) antibody (ab183319)

  • Datasheet
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  • Western blot - Anti-FMRP (phospho S499) antibody (ab183319)
  • ELISA - Anti-FMRP (phospho S499) antibody (ab183319)

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Primary

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-FMRP (phospho S499) antibody
    See all FMRP primary antibodies
  • Description

    Rabbit polyclonal to FMRP (phospho S499)
  • Host species

    Rabbit
  • Specificity

    ab183319 is specific for FMRP when phosphorylated at serine 499. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Sheep, Chicken, Cow, Dog, Human, Xenopus laevis, Non human primates, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Rat FMRP (phospho S499).
    Database link: Q80WE1

  • Positive control

    • Rat hippocampal lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.5
    Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab183319 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • RNAi
    • RISC

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human FMRP protein (ab132093)

Applications

Our Abpromise guarantee covers the use of ab183319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 67 kDa.

Target

  • Function

    Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
  • Tissue specificity

    Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
  • Involvement in disease

    Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
    Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
    Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities

    Belongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    modifications

    Phosphorylated on several serine residues.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession Q06787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2332 Human
    • Entrez Gene: 14265 Mouse
    • Entrez Gene: 24948 Rat
    • Entrez Gene: 259191 Zebrafish
    • Omim: 309550 Human
    • SwissProt: Q06787 Human
    • SwissProt: P35922 Mouse
    • SwissProt: Q80WE1 Rat
    • Unigene: 103183 Human
    • Unigene: 3451 Mouse
    • Unigene: 40595 Rat
    see all
  • Alternative names

    • FMR 1 antibody
    • Fmr1 antibody
    • Fmr1 gene antibody
    • FMR1_HUMAN antibody
    • FMRP antibody
    • Fragile X mental retardation 1 antibody
    • Fragile X mental retardation 1 protein antibody
    • Fragile X mental retardation protein 1 antibody
    • Fragile X mental retardation protein antibody
    • fragile X mental retardation syndrome-related protein 1 antibody
    • fragile X mental retardation, autosomal homolog 1 antibody
    • FRAXA antibody
    • fxr1 antibody
    • MGC87458 antibody
    • POF antibody
    • POF1 antibody
    • Protein FMR-1 antibody
    • Protein FMR1 antibody
    • wu:fb16f11 antibody
    • wu:fd18c10 antibody
    • zgc:66226 antibody
    see all

Images

  • Western blot - Anti-FMRP (phospho S499) antibody (ab183319)
    Western blot - Anti-FMRP (phospho S499) antibody (ab183319)
    All lanes : Anti-FMRP (phospho S499) antibody (ab183319) at 1/1000 dilution

    Lane 1 : Rat hippocampal lysate
    Lane 2 : Rat hippocampal lysate treated with Lambda Phosphatase (400 units / 100 µL lysate for 30 minutes)

    Predicted band size: 67 kDa

  • ELISA - Anti-FMRP (phospho S499) antibody (ab183319)
    ELISA - Anti-FMRP (phospho S499) antibody (ab183319)

    Serially diluted ab183319 was bound to immobilised phospho (ab48658) - or control (unmodified) peptides (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50,000 times) and signal was developed with TMB substrate

Protocols

  • Western blot protocols

Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Kute PM  et al. NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP. Mol Brain 12:65 (2019). Read more (PubMed: 31291981) »
    See 1 Publication for this product

    Publishing research using ab183319? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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