Anti-FMRP (phospho S499) antibody (ab183319)
Key features and details
- Rabbit polyclonal to FMRP (phospho S499)
- Suitable for: ELISA, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-FMRP (phospho S499) antibody
See all FMRP primary antibodies -
Description
Rabbit polyclonal to FMRP (phospho S499) -
Host species
Rabbit -
Specificity
ab183319 is specific for FMRP when phosphorylated at serine 499. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Sheep, Chicken, Cow, Dog, Human, Xenopus laevis, Non human primates, Zebrafish
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Immunogen
Synthetic peptide corresponding to Rat FMRP (phospho S499).
Database link: Q80WE1 -
Positive control
- Rat hippocampal lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab183319 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab183319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 67 kDa. |
Target
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Function
Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). -
Tissue specificity
Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. -
Involvement in disease
Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the FMR1 family.
Contains 2 KH domains. -
Post-translational
modificationsPhosphorylated on several serine residues. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 2332 Human
- Entrez Gene: 14265 Mouse
- Entrez Gene: 24948 Rat
- Entrez Gene: 259191 Zebrafish
- Omim: 309550 Human
- SwissProt: Q06787 Human
- SwissProt: P35922 Mouse
- SwissProt: Q80WE1 Rat
see all -
Alternative names
- FMR 1 antibody
- Fmr1 antibody
- Fmr1 gene antibody
see all
Images
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Western blot - Anti-FMRP (phospho S499) antibody (ab183319)All lanes : Anti-FMRP (phospho S499) antibody (ab183319) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate treated with Lambda Phosphatase (400 units / 100 µL lysate for 30 minutes)
Predicted band size: 67 kDa -
ELISA - Anti-FMRP (phospho S499) antibody (ab183319)
Datasheets and documents
References (1)
ab183319 has been referenced in 1 publication.
- Kute PM et al. NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP. Mol Brain 12:65 (2019). PubMed: 31291981
