Key features and details
- Rabbit polyclonal to FMRP (phospho S499)
- Suitable for: ELISA, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-FMRP (phospho S499) antibody
See all FMRP primary antibodies
DescriptionRabbit polyclonal to FMRP (phospho S499)
Specificityab183319 is specific for FMRP when phosphorylated at serine 499. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Sheep, Chicken, Cow, Dog, Human, Xenopus laevis, Non human primates, Zebrafish
Synthetic peptide corresponding to Rat FMRP (phospho S499).
Database link: Q80WE1
- Rat hippocampal lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab183319 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns
Our Abpromise guarantee covers the use of ab183319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 67 kDa.|
FunctionTranslation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
Tissue specificityHighest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
Involvement in diseaseDefects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the FMR1 family.
Contains 2 KH domains.
modificationsPhosphorylated on several serine residues.
Cellular localizationCytoplasm. Nucleus > nucleolus.
- Information by UniProt
- FMR 1 antibody
- Fmr1 antibody
- Fmr1 gene antibody
All lanes : Anti-FMRP (phospho S499) antibody (ab183319) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate treated with Lambda Phosphatase (400 units / 100 µL lysate for 30 minutes)
Predicted band size: 67 kDa
ab183319 has been referenced in 1 publication.
- Kute PM et al. NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP. Mol Brain 12:65 (2019). PubMed: 31291981