Anti-FMRP (phospho S499) antibody (ab48127)


  • Product name
    Anti-FMRP (phospho S499) antibody
    See all FMRP primary antibodies
  • Description
    Rabbit polyclonal to FMRP (phospho S499)
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human FMRP.

    (Peptide available as ab48658.)

  • Positive control
    • This antibody gave a positive signal in both HeLa (Human epithelial carcinoma cell line) and HeLa Nuclear Lysate as well as Mouse brain tissue lysate. It also gave a positive signal in FFPE human cerebral cortex tissue sections.



Our Abpromise guarantee covers the use of ab48127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24658146
ELISA Use at an assay dependent concentration.


  • Function
    Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
  • Tissue specificity
    Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells.
  • Involvement in disease
    Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
    Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
    Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities
    Belongs to the FMR1 family.
    Contains 2 KH domains.
  • Post-translational
    Phosphorylated on several serine residues.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FMR 1 antibody
    • Fmr1 antibody
    • Fmr1 gene antibody
    • FMR1_HUMAN antibody
    • FMRP antibody
    • Fragile X mental retardation 1 antibody
    • Fragile X mental retardation 1 protein antibody
    • Fragile X mental retardation protein 1 antibody
    • Fragile X mental retardation protein antibody
    • fragile X mental retardation syndrome-related protein 1 antibody
    • fragile X mental retardation, autosomal homolog 1 antibody
    • FRAXA antibody
    • fxr1 antibody
    • MGC87458 antibody
    • POF antibody
    • POF1 antibody
    • Protein FMR-1 antibody
    • Protein FMR1 antibody
    • wu:fb16f11 antibody
    • wu:fd18c10 antibody
    • zgc:66226 antibody
    see all


  • Serially diluted ab48127 was bound to immobilised phospho (ab48658) - or control (unmodified) peptides (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50,000 times) and signal was developed with TMB substrate

  • All lanes : Anti-FMRP (phospho S499) antibody (ab48127) at 2 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Mouse Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes
  • IHC image of FMRP (phospho S499) staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 48127, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-FMRP (phospho S499) antibody (ab48127) at 0.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 71 kDa

    Exposure time: 5 minutes


This product has been referenced in:
  • Lechpammer M  et al. Dysregulation of FMRP/mTOR Signaling Cascade in Hypoxic-Ischemic Injury of Premature Human Brain. J Child Neurol 31:426-32 (2016). Read more (PubMed: 26239490) »
  • Spencer KB  et al. FMRP Mediates Chronic Ethanol-Induced Changes in NMDA, Kv4.2, and KChIP3 Expression in the Hippocampus. Alcohol Clin Exp Res 40:1251-61 (2016). Read more (PubMed: 27147118) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you very much for your reply.

I'll be happy to send the blocking peptide. Do you have an order or PO number?

I look forward to hearing from you. Please let me know if there is anything else you need from me at this time.

Zebrafish Tissue lysate - whole (brain)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Jul 16 2015

Western blot
Zebrafish Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Abcam user community

Verified customer

Submitted Jul 16 2015

Thank you very much for your replies today.

I'm sending the peptide free of charge on the order ***, which should arrive within the next two days.

Please keep me updated about any new results using the blocking peptide, and let me k...

Read More

Thank you so much for sending the additional information and for your patience. I'm in the midst of a literature search but I will be back in touch this afternoon. Please let me know if you have any questions or need anything else in the meantime.

Thank you for your call yesterday and for sending the images.

I have a couple of additional questions. There are three blots showing results with hippocampus tissue- is this all tissue from the same animal, or are they different animals? Is t...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain)
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Jun 08 2012

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I am very pleased to hear you would like to accept our offer and test ab48127 in mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this ...

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Thank you for your review of ab48127, anti-FMRP (phospho S499), which I just published. The western blot result is disappointing, given the lack of a strong signal at the expected molecular weight of 71 kDa. I do not think the bands at 135 kDa or 125 ...

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