Key features and details
- Rabbit polyclonal to FN3K
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-FN3K antibody
See all FN3K primary antibodies
DescriptionRabbit polyclonal to FN3K
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Recombinant full length protein (His-tag) aa 1-309. Expressed in E.coli. N-terminal tag.
MEQLLRAELRTATLRAFGGPGAGCISEGRAYDTDAGPVFVKVNRRTQARQ MFEGEVASLEALRSTGLVRVPRPMKVIDLPGGGAAFVMEHLKMKSLSSQA SKLGEQMADLHLYNQKLREKLKEEENTVGRRGEGAEPQYVDKFGFHTVTC CGFIPQVNEWQDDWPTFFARHRLQAQLDLIEKDYADREARELWSRLQVKI PDLFCGLEIVPALLHGDLWSGNVAEDDVGPIIYDPASFYGHSEFELAIAL MFGGFPRSFFTAYHRKIPKAPGFDQRLLLYQLFNYLNHWNHFGREYRSPS LGTMRRLLK
Database link: Q9H479
- WB: Recombinant human FN3K protein; HeLa and HEK-293T cell lysates; Pig heart and brain lysates. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
RelevanceFN3K catalyzes phosphorylation of fructosamines formed by glycation, the non-enzymatic reaction of glucose with primary amines followed by Amadori re-arrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and lead to the de-glycation of fructoselysine and of glycated proteins.
- 2310074G21Rik antibody
- Fnsk antibody
- Fructosamine 3 kinase antibody
- RP23-293H17.4 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FN3K (green) using ab232766 at 20 μg/ml in ICC/IF. FITC-labeled secondary antibody.
All lanes : Anti-FN3K antibody (ab232766) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Pig heart lysate
Lane 4 : Pig brain lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Anti-FN3K antibody (ab232766) at 2 µg/ml + Recombinant human FN3K protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232766 has not yet been referenced specifically in any publications.