Anti-Toca-1 antibody (ab67310)
Key features and details
- Rabbit polyclonal to Toca-1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Toca-1 antibody -
Description
Rabbit polyclonal to Toca-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Toca-1 (C terminal). The immunogen is located within the last 50 amino acids (17 amino acid synthetic peptide) of TOCA-1.
Database link: Q5T0N5 -
Positive control
- WB: Mouse brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 70 kDa).
This application in validated in mouse samples. |
Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 70 kDa). This application in validated in mouse samples. |
Target
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Function
Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. -
Sequence similarities
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 214459 Mouse
- SwissProt: Q8K012 Mouse
- Unigene: 209491 Mouse
- Unigene: 470230 Mouse
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Alternative names
- C1orf39 antibody
- FBP1L_HUMAN antibody
- FNBP 1L antibody
see all
Images
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All lanes : Anti-Toca-1 antibody (ab67310) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate with no blocking peptide
Lane 2 : Mouse brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 70 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab67310 has been referenced in 1 publication.
- Drummond ML et al. Actin polymerization controls cilia-mediated signaling. J Cell Biol 217:3255-3266 (2018). PubMed: 29945904