Key features and details
- Rabbit polyclonal to FNBP3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FNBP3 antibody
DescriptionRabbit polyclonal to FNBP3
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human rectum tissue, RT-4 and U-251 MG cell lysate, U-2 OS cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 109 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionBinds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). May be involved in pre-mRNA splicing.
Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed.
Sequence similaritiesBelongs to the PRPF40 family.
Contains 5 FF domains.
Contains 2 WW domains.
DomainThe WW domains are essential for localization to nuclear speckles.
Cellular localizationNucleus speckle. Nucleus matrix. Colocalizes with AKAP8L in the nuclear matrix.
- Information by UniProt
- Fas ligand associated factor 1 antibody
- Fas ligand-associated factor 1 antibody
- FBP 11 antibody
Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling FNBP3 with ab204371 at 1/200 dilution.
All lanes : Anti-FNBP3 antibody (ab204371) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Developed using the ECL technique.
Predicted band size: 109 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling FNBP3 with ab204371 at 4 µg/mL (green).
ab204371 has been referenced in 1 publication.
- Huo Z et al. PRPF40A as a potential diagnostic and prognostic marker is upregulated in pancreatic cancer tissues and cell lines: an integrated bioinformatics data analysis. Onco Targets Ther 12:5037-5051 (2019). PubMed: 31303762