Anti-FNBP3 antibody (ab204371)
Key features and details
- Rabbit polyclonal to FNBP3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FNBP3 antibody -
Description
Rabbit polyclonal to FNBP3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human FNBP3 aa 200-300.
Database link: O75400 -
Positive control
- IHC-P: Human rectum tissue; WB: K562 and MCF7 cell lysates; ICC: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 109 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 109 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). May be involved in pre-mRNA splicing. -
Tissue specificity
Expressed in the brain cortex (at protein level). Widely expressed. -
Sequence similarities
Belongs to the PRPF40 family.
Contains 5 FF domains.
Contains 2 WW domains. -
Domain
The WW domains are essential for localization to nuclear speckles. -
Cellular localization
Nucleus speckle. Nucleus matrix. Colocalizes with AKAP8L in the nuclear matrix. - Information by UniProt
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Database links
- Entrez Gene: 55660 Human
- Entrez Gene: 56194 Mouse
- Entrez Gene: 295607 Rat
- Omim: 612941 Human
- SwissProt: O75400 Human
- SwissProt: Q9R1C7 Mouse
- Unigene: 643580 Human
- Unigene: 257474 Mouse
see all -
Alternative names
- Fas ligand associated factor 1 antibody
- Fas ligand-associated factor 1 antibody
- FBP 11 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling FNBP3 with ab204371 at 1/200 dilution.
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All lanes : Anti-FNBP3 antibody (ab204371) at 0.4 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 109 kDa -
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling FNBP3 with ab204371 at 4 µg/mL (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab204371 has been referenced in 1 publication.
- Huo Z et al. PRPF40A as a potential diagnostic and prognostic marker is upregulated in pancreatic cancer tissues and cell lines: an integrated bioinformatics data analysis. Onco Targets Ther 12:5037-5051 (2019). PubMed: 31303762