Overview

  • Product name

    Anti-FNDC5 antibody [EPR12209]
    See all FNDC5 primary antibodies
  • Description

    Rabbit monoclonal [EPR12209] to FNDC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FNDC5 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NAU1
    (Peptide available as ab204133)

  • Positive control

    • WB: Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869), Human fetal, mouse and rat muscle tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12209
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa.Can be blocked with FNDC5 peptide (ab204133).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    Images

    • Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution (purified) + Human fetal muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution (purified) + Mouse muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/5000 dilution (purified) + Rat muscle at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    This product has been referenced in:

    • Bi J  et al. Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress. Redox Biol 20:296-306 (2019). Read more (PubMed: 30388684) »
    • Yu Q  et al. FNDC5/Irisin inhibits pathological cardiac hypertrophy. Clin Sci (Lond) 133:611-627 (2019). Read more (PubMed: 30782608) »
    See all 14 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12 cells)
    Gel Running Conditions
    Reduced Denaturing (11% SDS-PAGE)
    Loading amount
    30 µg
    Treatment
    2mM Metformin, 1mM AICAR, 1ng/ml TGF-beta1 for 24hr
    Specification
    C2C12 cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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