RecombinantRabMAb

Recombinant Anti-FNDC5 antibody [EPR12209] (ab174833)

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Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
  • Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
  • Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
  • Anti-FNDC5 antibody [EPR12209] (ab174833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12209] to FNDC5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FNDC5 antibody [EPR12209]
    See all FNDC5 primary antibodies

  • Description

    Rabbit monoclonal [EPR12209] to FNDC5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FNDC5 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NAU1
    (Peptide available as ab204133)

  • Positive control

    • WB: Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869), Human fetal, mouse and rat muscle tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12209

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa.Can be blocked with FNDC5 peptide (ab204133).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.

    • Target

    Images

    • Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
      Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
      Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution (purified) + Human fetal muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
      Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
      Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/1000 dilution (purified) + Mouse muscle tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
      Western blot - Anti-FNDC5 antibody [EPR12209] (ab174833)
      Anti-FNDC5 antibody [EPR12209] (ab174833) at 1/5000 dilution (purified) + Rat muscle at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-FNDC5 antibody [EPR12209] (ab174833)
      Anti-FNDC5 antibody [EPR12209] (ab174833)

    References (24)

    Publishing research using ab174833? Please let us know so that we can cite the reference in this datasheet.

    ab174833 has been referenced in 24 publications.

    • Bi J  et al. Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury. Oxid Med Cell Longev 2020:6946037 (2020). PubMed: 31976032
    • Nie Y  et al. Cleavage of FNDC5 and insights into its maturation process. Mol Cell Endocrinol 510:110840 (2020). PubMed: 32360564
    • Aslan G  et al. Ischemic postconditioning reduced myocardial ischemia-reperfusion injury: The roles of melatonin and uncoupling protein 3. Anatol J Cardiol 23:19-27 (2020). PubMed: 31911566
    • Zhang J  et al. Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis. Lipids Health Dis 19:39 (2020). PubMed: 32169116
    • Chen SQ  et al. Icariin induces irisin/FNDC5 expression in C2C12 cells via the AMPK pathway. Biomed Pharmacother 115:108930 (2019). PubMed: 31055234
    View all Publications for this product

    Customer reviews and Q&As

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    Western blot abreview for Anti-FNDC5 antibody [EPR12209]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12 cells)
    Gel Running Conditions
    Reduced Denaturing (11% SDS-PAGE)
    Loading amount
    30 µg
    Treatment
    2mM Metformin, 1mM AICAR, 1ng/ml TGF-beta1 for 24hr
    Specification
    C2C12 cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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