Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 cells)
Gel Running Conditions
Reduced Denaturing (11% SDS-PAGE)
Loading amount
30 µg
Treatment
2mM Metformin, 1mM AICAR, 1ng/ml TGF-beta1 for 24hr
Specification
C2C12 cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-rabbit IgG(H+L) HRP antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal Pico Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 24 kDa
Abcam user community
Verified customer
Submitted Jan 18 2017