Overview

  • Product name

    Anti-FNIP1 antibody [EPNCIR107]
    See all FNIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR107] to FNIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FNIP1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q8TF40

  • Positive control

    • FNIP transfected 293T lysate, treated with doxorubicin; Human liver tissue.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Marston Linehan. View antibodies from NCI Center for Cancer Research Collaboration.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR107
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 131 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
    • Cellular localization

      Cytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
    • Database links

    • Alternative names

      • KIAA1961 antibody
      • FLCN-interacting protein antibody
      • FNIP1 antibody
      • Folliculin interacting protein 1 antibody

    Images

    • All lanes : Anti-FNIP1 antibody [EPNCIR107] (ab134969) at 1/1000 dilution

      Lane 1 : FNIP transfected
      293T lysate, untreated
      Lane 2 : FNIP transfected
      293T lysate, treated with doxorubicin

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 131 kDa

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling FNIP1 with ab134969 at 1/50 dilution.
    • Immunohistochemical analysis using ab134969 showing positive staining in Skeletal muscle tissue.

    • Immunohistochemical analysis using ab134969 showing positive staining in Normal kidney tissue.

    • Immunohistochemical analysis using ab134969 showing positive staining in Thyroid gland papillary carcinoma tissue.

    References

    This product has been referenced in:

    • Nagashima K  et al. Nutrient-induced FNIP degradation by SCFß-TRCP regulates FLCN complex localization and promotes renal cancer progression. Oncotarget 8:9947-9960 (2017). Read more (PubMed: 28039480) »
    • Petit CS  et al. Recruitment of folliculin to lysosomes supports the amino acid-dependent activation of Rag GTPases. J Cell Biol 202:1107-22 (2013). Read more (PubMed: 24081491) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134969.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up