Product nameAnti-FNIP1 antibody [EPNCIR107]
See all FNIP1 primary antibodies
DescriptionRabbit monoclonal [EPNCIR107] to FNIP1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human FNIP1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q8TF40
- FNIP transfected 293T lysate, treated with doxorubicin; Human liver tissue.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Marston Linehan. View antibodies from NCI Center for Cancer Research Collaboration.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 131 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
RelevanceMay be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
Cellular localizationCytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
- KIAA1961 antibody
- FLCN-interacting protein antibody
- FNIP1 antibody
- Folliculin interacting protein 1 antibody
All lanes : Anti-FNIP1 antibody [EPNCIR107] (ab134969) at 1/1000 dilution
Lane 1 : FNIP transfected
293T lysate, untreated
Lane 2 : FNIP transfected
293T lysate, treated with doxorubicin
Lysates/proteins at 10 µg/ml per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 131 kDa
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling FNIP1 with ab134969 at 1/50 dilution.
Immunohistochemical analysis using ab134969 showing positive staining in Skeletal muscle tissue.
Immunohistochemical analysis using ab134969 showing positive staining in Normal kidney tissue.
Immunohistochemical analysis using ab134969 showing positive staining in Thyroid gland papillary carcinoma tissue.
This product has been referenced in:
- Nagashima K et al. Nutrient-induced FNIP degradation by SCFß-TRCP regulates FLCN complex localization and promotes renal cancer progression. Oncotarget 8:9947-9960 (2017). Read more (PubMed: 28039480) »
- Petit CS et al. Recruitment of folliculin to lysosomes supports the amino acid-dependent activation of Rag GTPases. J Cell Biol 202:1107-22 (2013). Read more (PubMed: 24081491) »