Overview

  • Product name

    Anti-FNIP1 antibody [EPR20832]
    See all FNIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20832] to FNIP1
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse FNIP1 aa 1-100. The exact sequence is proprietary.
    Database link: Q68FD7

  • Positive control

    • WB: Mouse muscle and testis tissue lysates; L-929 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20832
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).

Target

  • Relevance

    May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
  • Cellular localization

    Cytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
  • Database links

  • Alternative names

    • KIAA1961 antibody
    • FLCN-interacting protein antibody
    • FNIP1 antibody
    • Folliculin interacting protein 1 antibody

Images

  • All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 130 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 1 minute; Lane 2: 70 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174). The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Lane 2 was developed using a high sensitivity ECL substrate. Expression levels of the target protein vary with sample type and some optimisation may be required

  • All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/500 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : FNIP1 knockout mouse muscle lysate

    Lysates/proteins at 75 µg per lane.

    Secondary
    All lanes : Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/7500 dilution

    Predicted band size: 130 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 15 minutes


    Blocking buffer: 7% non-fat milk diluted in TBS-T.

    Dilution buffer: 1% BSA diluted in TBS.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174).

    The image was kindly provided by our collaborator Dr. Zhenji Gan, Nanjing University.

    The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Expression levels of the target protein vary with sample type and some optimisation may be required.

References

ab215725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215725.
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