Product nameAnti-FNIP1 antibody [EPR20832] - BSA and Azide free
See all FNIP1 primary antibodies
DescriptionRabbit monoclonal [EPR20832] to FNIP1 - BSA and Azide free
SpecificityExpression levels of the target protein vary with sample type and some optimisation may be required.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse FNIP1 aa 1-100. The exact sequence is proprietary.
Database link: Q68FD7
- WB: Mouse muscle lysate.
Ab236547 is the carrier-free version of ab215725. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab236547 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab236547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).|
RelevanceMay be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
Cellular localizationCytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
- KIAA1961 antibody
- FLCN-interacting protein antibody
- FNIP1 antibody
- Folliculin interacting protein 1 antibody
All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/500 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : FNIP1 knockout mouse muscle lysate
Lysates/proteins at 75 µg per lane.
All lanes : Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/7500 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
Blocking buffer: 7% non-fat milk diluted in TBS-T.
Dilution buffer: 1% BSA diluted in TBS.
The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174).
The image was kindly provided by our collaborator Dr. Zhenji Gan, Nanjing University.
The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Expression levels of the target protein vary with sample type and some optimisation may be required.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215725).
ab236547 has not yet been referenced specifically in any publications.