Recombinant
RabMAb

Recombinant Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)

Overview

  • Product name

    Anti-FNIP1 antibody [EPR20832] - BSA and Azide free
    See all FNIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20832] to FNIP1 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse FNIP1 aa 1-100. The exact sequence is proprietary.
    Database link: Q68FD7

  • Positive control

    • WB: Mouse muscle lysate.
  • General notes

    Ab236547 is the carrier-free version of ab215725. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236547 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20832
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).

Target

  • Relevance

    May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
  • Cellular localization

    Cytoplasm. Note: Co-localizes with FLCN in the cytoplasm.
  • Database links

  • Alternative names

    • KIAA1961 antibody
    • FLCN-interacting protein antibody
    • FNIP1 antibody
    • Folliculin interacting protein 1 antibody

Images

  • All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/500 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : FNIP1 knockout mouse muscle lysate

    Lysates/proteins at 75 µg per lane.

    Secondary
    All lanes : Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/7500 dilution

    Predicted band size: 130 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 minutes


    Blocking buffer: 7% non-fat milk diluted in TBS-T.

    Dilution buffer: 1% BSA diluted in TBS.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174).

    The image was kindly provided by our collaborator Dr. Zhenji Gan, Nanjing University.

    The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Expression levels of the target protein vary with sample type and some optimisation may be required.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215725).

     

References

ab236547 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236547.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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