Key features and details
- Rabbit polyclonal to FNTA - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FNTA antibody - C-terminal
See all FNTA primary antibodies
DescriptionRabbit polyclonal to FNTA - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Common marmoset
- HeLa, 293T and Jurkat whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab186136 was affinity purified using an epitope specific to FNTA immobilized on a solid support.
Our Abpromise guarantee covers the use of ab186136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCatalyzes the transfer of a farnesyl or geranyl-geranyl moiety from farnesyl or geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. The alpha subunit is thought to participate in a stable complex with the substrate. The beta subunit binds the peptide substrate. Through RAC1 prenylation and activation may positively regulate neuromuscular junction development downstream of MUSK.
Sequence similaritiesBelongs to the protein prenyltransferase subunit alpha family.
Contains 5 PFTA repeats.
modificationsPhosphorylated. Phosphorylation is mediated by MUSK upon AGRIN stimulation and results in the activation of FNTA.
- Information by UniProt
- CAAX farnesyltransferase alpha subunit antibody
- CAAX farnesyltransferase subunit alpha antibody
- EC 188.8.131.52 antibody
Detection of FNTA in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab186136 at 6 µg per reaction for IP (Lane 1). For WB detection, ab186136 was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-FNTA antibody - C-terminal (ab186136) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes
ab186136 has been referenced in 1 publication.
- Mohammad G et al. Functional Regulation of an Oxidative Stress Mediator, Rac1, in Diabetic Retinopathy. Mol Neurobiol 56:8643-8655 (2019). PubMed: 31300985