Product nameAnti-FNTB antibody
See all FNTB primary antibodies
DescriptionRabbit polyclonal to FNTB
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human FNTB aa 1-300.
Database link: P49356
- WB: HeLa, CEM, HepG2, SH-SY5Y, U-87 MG, A-375 and A549 whole cell lysates. IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 49 kDa.|
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
FunctionCatalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.
Sequence similaritiesBelongs to the protein prenyltransferase subunit beta family.
Contains 5 PFTB repeats.
- Information by UniProt
- CAAX farnesyltransferase subunit beta antibody
- EC 126.96.36.199 antibody
- EC=188.8.131.52 antibody
All lanes : Anti-FNTB antibody (ab236649) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : CEM whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 6 : A-375 (human malignant melanoma cell line) whole cell lysate
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Paraffin-embedded human tonsil tissue stained for FNTB using ab236649 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FNTB (green) using ab236649 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
FNTB was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236649 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate.
Lane 2: ab236649 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
ab236649 has not yet been referenced specifically in any publications.