Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4707] to FNTB
- Suitable for: WB, IP, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-FNTB antibody [EPR4707]
See all FNTB primary antibodies
DescriptionRabbit monoclonal [EPR4707] to FNTB
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Unsuitable for: ICC
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues at the C-terminus of Human FNTB (P49356)
- JAR, A431, U87-MG and A375 cell lysates, Human breast tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 49 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.
Sequence similaritiesBelongs to the protein prenyltransferase subunit beta family.
Contains 5 PFTB repeats.
- Information by UniProt
- CAAX farnesyltransferase subunit beta antibody
- EC 18.104.22.168 antibody
- EC=22.214.171.124 antibody
All lanes : Anti-FNTB antibody [EPR4707] (ab109625) at 1/10000 dilution
Lane 1 : JAR cell lysate
Lane 2 : A431 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109625 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109625 has been referenced in 2 publications.
- Pinner AL et al. Protein expression of prenyltransferase subunits in postmortem schizophrenia dorsolateral prefrontal cortex. Transl Psychiatry 10:3 (2020). PubMed: 32066669
- Liang J et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). PubMed: 29670091