Anti-FOG1 antibody (ab84876)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FOG1 antibody
See all FOG1 primary antibodies -
Description
Rabbit polyclonal to FOG1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to a region between residue 175 and 225 of human FOG1 (accession number: NP_722520.2)
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Positive control
- Human Controls: Breast Carcinoma, Hodgkins Lymphoma, Prostate Carcinoma
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to FOG1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab84876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2. -
Tissue specificity
Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen. -
Sequence similarities
Belongs to the FOG (Friend of GATA) family.
Contains 4 C2H2-type zinc fingers.
Contains 5 C2HC-type zinc fingers. -
Domain
The CCHC-type zinc fingers 1, 5, 6 and 9 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC-type zinc finger is essential for the interaction with GATA-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 161882 Human
- Omim: 601950 Human
- SwissProt: Q8IX07 Human
- Unigene: 632218 Human
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Alternative names
- FOG-1 antibody
- FOG1_HUMAN antibody
- Friend of GATA 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOG1 antibody (ab84876)Detection of Human FOG1 in formalin-fixed paraffin-embedded section of human Hodgkin's Lymphoma by Immunohistochemistry using ab84876 at a dilution of 1:250. Detection: DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOG1 antibody (ab84876)Detection of Human FOG1 in formalin-fixed paraffin-embedded section of human breast carcinoma by Immunohistochemistry using ab84876 at a dilution of 1:250. Detection: DAB staining.
Protocols
Datasheets and documents
References
ab84876 has not yet been referenced specifically in any publications.
