Anti-FOG1 antibody (ab84876)
Key features and details
- Rabbit polyclonal to FOG1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOG1 antibody
See all FOG1 primary antibodies -
Description
Rabbit polyclonal to FOG1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to a region between residue 175 and 225 of human FOG1 (accession number: NP_722520.2)
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Positive control
- Human Controls: Breast Carcinoma, Hodgkins Lymphoma, Prostate Carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to FOG1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| All applications |
Chimpanzee
Gorilla
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
| Notes |
|---|
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2. -
Tissue specificity
Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen. -
Sequence similarities
Belongs to the FOG (Friend of GATA) family.
Contains 4 C2H2-type zinc fingers.
Contains 5 C2HC-type zinc fingers. -
Domain
The CCHC-type zinc fingers 1, 5, 6 and 9 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC-type zinc finger is essential for the interaction with GATA-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 161882 Human
- Omim: 601950 Human
- SwissProt: Q8IX07 Human
- Unigene: 632218 Human
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Alternative names
- FOG-1 antibody
- FOG1_HUMAN antibody
- Friend of GATA 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOG1 antibody (ab84876)Detection of Human FOG1 in formalin-fixed paraffin-embedded section of human Hodgkin's Lymphoma by Immunohistochemistry using ab84876 at a dilution of 1:250. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOG1 antibody (ab84876)Detection of Human FOG1 in formalin-fixed paraffin-embedded section of human breast carcinoma by Immunohistochemistry using ab84876 at a dilution of 1:250. Detection: DAB staining.
Datasheets and documents
References (0)
ab84876 has not yet been referenced specifically in any publications.
