Anti-Folate Binding Protein / FBP antibody [LK26] (ab3361)

Overview

  • Product name
    Anti-Folate Binding Protein / FBP antibody [LK26]
    See all Folate Binding Protein / FBP primary antibodies
  • Description
    Mouse monoclonal [LK26] to Folate Binding Protein / FBP
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, IP, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Folate Binding Protein/ FBP. Gestational choriocarcinoma cell line, Lu-75.

  • Positive control
    • Frozen Placenta or Ovarian Carcinoma (Negative control: Mouse serum or IgG1 isotype-matched negative control antibody, diluted to the same concentration as the primary antibody, should also be included in each run.)
  • General notes

    Previously labelled as Folate Binding Protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    LK26
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/40.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 19567824

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration. PubMed: 19636639
WB 1/500. PubMed: 18553957

Target

  • Function
    Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • Tissue specificity
    Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
  • Involvement in disease
    Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
  • Sequence similarities
    Belongs to the folate receptor family.
  • Post-translational
    modifications
    Eight disulfide bonds are present.
    The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
  • Cellular localization
    Cell membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • adult antibody
    • Adult folate binding protein antibody
    • Adult folate-binding protein antibody
    • FBP antibody
    • Folate Binding Protein antibody
    • Folate Receptor 1 Adult antibody
    • Folate receptor 1 antibody
    • Folate Receptor 1 Precursor antibody
    • Folate receptor adult antibody
    • Folate receptor alpha antibody
    • Folate receptor antibody
    • FOLR antibody
    • FOLR1 antibody
    • FOLR1_HUMAN antibody
    • FR alpha antibody
    • FR-alpha antibody
    • FRalpha antibody
    • KB cells FBP antibody
    • MOV18 antibody
    • Ovarian cancer associated antigen antibody
    • Ovarian tumor associated antigen antibody
    • Ovarian tumor associated antigen MOv18 antibody
    • Ovarian tumor-associated antigen MOv18 antibody
    see all

Images

  • ab3361 staining frozen placenta in IHC-F.

References

This product has been referenced in:
  • Siwowska K  et al. Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization. Pharmaceuticals (Basel) 10:N/A (2017). Read more (PubMed: 28809784) »
  • Xie M  et al. Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine. Int J Nanomedicine 8:2443-51 (2013). Human . Read more (PubMed: 23874095) »

See all 7 Publications for this product

Customer reviews and Q&As

It will depend on how you want to use the antibody and how much is required for each test . I updated the ab2107 datasheet to show suggested dilution ranges for the applications that are listed on the datasheet.

WB: 1/500 - 1/5000.
IP: ...

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Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement with ab125030, the order number is *******.

To check the status of the order please contact our Customer Service...

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Gracias por tu respuesta.

Si te parece puedo enviaros para que probéis un anticuerpo diferente contra la misma proteína.

Echarle un vistazo a los otros productos que tenemos disponibles contra Folate Binding Protein va...

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Muchas gracias por mandarnos el cuestionario relleno.

Para intentar optimizar los resultados del anticuerpo hay una serie de sugerencias que me gustaría hacer. De cualquier manera, al estar al anticuerpo bajo garantía en caso de...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP-1)
Permeabilization
No
Specification
THP-1
Blocking step
Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2008

Thank you for your enquiry. Ab3361 was generated using gestational choriocarcinoma cell line, Lu-75, as the immunogen. However, we have not conducted testing to determine whether the antibody is specific for one or the other subtype of the receptor. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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