Key features and details
- Rabbit polyclonal to Folate Binding Protein/FBP
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Folate Binding Protein/FBP antibody
See all Folate Binding Protein/FBP primary antibodies
DescriptionRabbit polyclonal to Folate Binding Protein/FBP
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
- IHC-P: Human lung and kidney tissues. WB: HeLa cell lysate. Flow: HeLa cells.
Previously labelled as Folate Binding Protein
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/100.|
|WB||1/1000. Predicted molecular weight: 30 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
Tissue specificityExclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
Involvement in diseaseDefects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
Sequence similaritiesBelongs to the folate receptor family.
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
Cellular localizationCell membrane. Secreted.
- Information by UniProt
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
Flow cytometric analysis of HeLA (human epithelial cell line from cervix adenocarcinoma) cells labeling Folate Binding Protein/FBP with ab230469at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed, paraffin-embedded human lung tissue stained for Folate Binding Protein/FBP antibody using ab230469 at 1/10 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Folate Binding Protein/FBP antibody using ab230469 at 1/10 dilution in immunohistochemical analysis.
Anti-Folate Binding Protein/FBP antibody (ab230469) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg
goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230469 has not yet been referenced specifically in any publications.