Key features and details
- Rabbit polyclonal to Folate Binding Protein/FBP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Folate Binding Protein/FBP antibody
See all Folate Binding Protein/FBP primary antibodies
DescriptionRabbit polyclonal to Folate Binding Protein/FBP
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken
- WB: Mouse cerebellum tissue lysate, JAR and HeLa cell lysates. ICC/IF: HepG2 cells.
Previously labelled as Folate Binding Protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab67422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). Please note, we have been advised by some customers that ab67422 is unable to detect the human version of this protein in western blot. Please contact our scientific support services if you have any queries regarding this antibody.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionBinds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
Tissue specificityExclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
Involvement in diseaseDefects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
Sequence similaritiesBelongs to the folate receptor family.
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
Cellular localizationCell membrane. Secreted.
- Information by UniProt
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
All lanes : Anti-Folate Binding Protein/FBP antibody (ab67422) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FOLR1 knockout HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : MDA-MB-231 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Lanes 1-4: Merged signal (red and green). Green - ab67422 observed at 38 kDa. Red - loading control, ab7291 observed at 52 kDa.
ab67422 Anti-Folate Binding Protein/FBP antibody was shown to specifically react with Folate Binding Protein/FBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264921 (knockout cell lysate ab257270) was used. Wild-type and Folate Binding Protein/FBP knockout samples were subjected to SDS-PAGE. ab67422 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Anti-Folate Binding Protein/FBP antibody (ab67422) at 1/1 dilution + Cerebellum Mouse Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible cleavage fragment), 22 kDa (possible cleavage fragment)
We hypothesize that the 28, 22 and 16 kDa bands represent the propeptide with signal sequence, propeptide without signal sequence and mature protein, respectively.
IHC image of Folate Binding Protein/FBP staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67422, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab67422 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67422 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab67422 has been referenced in 4 publications.
- Martin JB et al. Folic acid modifies the shape of epithelial cells during morphogenesis via a Folr1 and MLCK dependent mechanism. Biol Open 8:N/A (2019). PubMed: 30670450
- Siwowska K et al. Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization. Pharmaceuticals (Basel) 10:N/A (2017). PubMed: 28809784
- Meredith M et al. Growing Mouse Oocytes Transiently Activate Folate Transport via Folate Receptors As They Approach Full Size. Biol Reprod 94:125 (2016). PubMed: 27122634
- Ocak M et al. Folate receptor-targeted multimodality imaging of ovarian cancer in a novel syngeneic mouse model. Mol Pharm 12:542-53 (2015). PubMed: 25536192