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Hello, I would like to use ab67422 antibody to detect Folate Receptor 1 in Xenopus laevis samples. However, I need the exact sequence of the peptide you used as immunogen to know the extent of homology. In the case if you can't give me that information you could just e-mail me how many consecutive amino acids in the immunogen are homologous to Xenopus laevis FOLR1 (NP_001086114). Thank you!
Asked on Aug 10 2011
Thank you for your enquiry. I have also run a BLAST homology and the results is as below. gi|148225128|ref|NP_001086114.1| folate receptor 1 (adult) [Xenopus laevis] gi|49257600|gb|AAH74206.1| MGC82130 protein [Xenopus laevis] Length=243 GENE ID: 444543 folr1 | folate receptor 1 (adult) [Xenopus laevis] (10 or fewer PubMed links) Score = 32.3 bits (72), Expect = 0.35, Method: Compositional matrix adjust. Identities = 12/16 (75%), Positives = 14/16 (88%), Gaps = 0/16 (0%) It seems that the percentage of homology is 75%. We can usually expect positive reactivity if the homology is more than 90% but having a low homology percentage does not necessary mean no cross reactivity whatsoever. This is simply a way to check for suitability. I hope the information above will be helpful. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!
Answered on Aug 10 2011