Recombinant Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281291-anti-folate-binding-protein-fbp-antibody-epr20277-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20277] to Folate Binding Protein/FBP
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Folate Binding Protein/FBP antibody [EPR20277]
See all Folate Binding Protein/FBP primary antibodies -
Description
Rabbit monoclonal [EPR20277] to Folate Binding Protein/FBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal kidney, mouse kidney, and rat kidney tissue lysates and JAR, and HeLa cell lysates. IHC-P: Human kidney, ovarian cancer and lung cancer tissues; mouse kidney tissue; rat kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20277 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa). |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
Tissue specificity
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
Involvement in disease
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2348 Human
- Entrez Gene: 171049 Rat
- Omim: 136430 Human
- SwissProt: P15328 Human
- SwissProt: P35846 Mouse
- Unigene: 73769 Human
- Unigene: 2135 Mouse
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Alternative names
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, ready to use). Cytoplasmic staining on human kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051, ready to use).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-383510-anti-folate-binding-proteinfbp-antibody-epr20277-western-blot-jar-mda-mb-231.jpg)
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)All lanes : Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FOLR1 knockout HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : MDA-MB-231 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab221543 observed at 38 kDa. Red - loading control, ab7291 observed at 52 kDa.
ab221543 Anti-Folate Binding Protein/FBP antibody [EPR20277] was shown to specifically react with Folate Binding Protein/FBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264921 (knockout cell lysate ab257270) was used. Wild-type and Folate Binding Protein/FBP knockout samples were subjected to SDS-PAGE. ab221543 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281297-anti-folate-binding-protein-fbp-antibody-epr20277-western-blot.jpg)
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)All lanes : Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/5000 dilution
Lane 1 : GST-His-tagged human recombinant FOLR1 (aa 25-160), 20 ng
Lane 2 : His-tagged human recombinant FOLR2 (aa 24-154), 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Exposure time:
Lanes 1-2: 5 seconds
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![Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281298-anti-folate-binding-protein-fbp-antibody-epr20277-western-blot.jpg)
Western blot - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)All lanes : Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543) at 1/1000 dilution
Lane 1 : JAR (human placenta choriocarcinoma epithelial cells) whole cell lysate at 20 µg
Lane 2 : Human fetal kidney lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : Rat kidney lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cells) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Exposure times:
Lane 1:10 seconds
Lane 2: 30 seconds
Lanes 3-4: 5 seconds
Lane 5: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281292-anti-folate-binding-protein-fbp-antibody-epr20277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Mainly membranous staining on tumor cells of human ovarian cancer (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281293-anti-folate-binding-protein-fbp-antibody-epr20277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Cytoplasmic staining on tumor cells of human lung cancer (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281294-anti-folate-binding-protein-fbp-antibody-epr20277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Positive staining on mouse kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281295-anti-folate-binding-protein-fbp-antibody-epr20277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). Positive staining on rat kidney (PMID: 22204844). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-281296-anti-folate-binding-protein-fbp-antibody-epr20277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)Negative control:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Folate Binding Protein/FBP with ab221543 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97501, ready to use). No staining on human tonsil (PMID: 22547449). Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)](https://www.abcam.com/ps/products/221/ab221543/Images/ab221543-13-benefits-of-recombinant-antibodies.png)
Anti-Folate Binding Protein/FBP antibody [EPR20277] (ab221543)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab221543 has been referenced in 4 publications.
- Furugen A et al. Effects of valproate, an HDAC inhibitor, on the expression of folate carriers and folate metabolism-related genes in the placenta of rats. Drug Metab Pharmacokinet 40:100409 (2021). PubMed: 34293696
- Wang Y et al. Decitabine Sensitizes the Radioresistant Lung Adenocarcinoma to Pemetrexed Through Upregulation of Folate Receptor Alpha. Front Oncol 11:668798 (2021). PubMed: 34079760
- Maniati E et al. Mouse Ovarian Cancer Models Recapitulate the Human Tumor Microenvironment and Patient Response to Treatment. Cell Rep 30:525-540.e7 (2020). PubMed: 31940494
- Jin YH et al. Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer. Cell Physiol Biochem 49:271-281 (2018). PubMed: 30138940